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MCQ on Microscopy Pdf

MCQ on Microscopy with Answers: 

  • A microscope is an instrument that can be used to observe small objects, even cells that are too small to be seen by the naked eye.
  • It is mainly used to amplify tiny objects into instruments that can be seen by the naked eye.
  • The ability of a microscope to distinguish two adjacent points as distinct and separate is known as resolving power. Resolving power is a function of the wavelength of light used and the numerical aperture (NA) of the lens system.
  • A compound microscope contains three separate lens systems. The condenser lens is placed between the light source and the specimen and it gathers and focuses the light rays in the plane of the microscopic field to view the specimen.
  • The image formed by an objective of a compound microscope is real and enlarged. 
  • The electron microscope is the most powerful.
  • The principle of an electron microscope is the same as that of an optical microscope, except that the electrons have shorter wavelengths and can see smaller objects.


Microscope MCQ Questions with Answers: 

1. What is a microscope?

(1) A microscope is an instrument that can be used to observe small objects, even cells.

(2) A microscope is a laboratory instrument used to examine objects that are too small to be seen by the naked eye.

(3) Both of these

(4) None of these

Answer: 3


2. Which microscope is more powerful?

(1) Electron microscope

(2) Compound Microscope

(3) Light Microscope

(4) Simple Microscope

Answer: 1


3. What is the function of the diaphragm in a microscope?

(1) Diaphragm controls the angle of the light cone reaching the specimen.

(2) Controls only the width of the bundle of light rays reaching the condenser.

(3) Both of these

(4) None of these

Answer: 3


4. The human eye’s unaided resolving power is_______

(1) 1 cm

(2) 100um

(3) 200 nm

(4) 400 nm

Answer: 2


5. The image formed by an objective of a compound microscope is

(1) Virtual and enlarged

(2) Virtual and diminished

(3) Real and diminished

(4) Real and enlarged

Answer: 4


6. Who was the first to describe “cells” in dead cork tissue?

(1) Hans Janssen

(2) Zaccharias Janssen

(2) Antonie van Leeuwenhoek

(2) Robert Hooke

Answer: 4


7. To view live cells, which of the following is used?

(1) SEM

(2) TEM

(3) Phase contrast microscope

(4) All of these

Answer: 3


8. In light microscopy, which of the following is used as fixatives before the staining technique?

(1) Osmic acid

(2) Glutaraldehyde

(3) Heat

(4) Osmic acid, glutaraldehyde, heat

Answer: 3


9. What is the resolution of a simple microscope?

(1) The simple microscope has a resolution of 10.

(2) The simple microscope has a resolution of 100

(3) The simple microscope has a resolution of 1000

(4) None of these

Answer: 1


10. Electron Microscope can give a magnification up to ___________

(1) 400,000X

(2) 100,000X

(3) 15000X

(4) 100X

Answer: 1


11. The refractive index of air is_______

(1) 0.50

(2) 0.75

(3) 1.00

(4) 1.25

Answer: 3


12. What is one difference between specimen preparation for a transmission electron microscope (TEM) and preparation for a scanning electron microscope (SEM)?

(1) Only the TEM specimen requires sputter coating.

(2) Only the SEM specimen requires sputter-coating.

(3) Only the TEM specimen must be dehydrated.

(4) Only the SEM specimen must be dehydrated.

Answer: 2


13. What is the focal length of a compound microscope?

(1) 0.95 cm and 5 cm

(2) 95 cm and 5 cm

(3) 0.9 cm and 0.5 cm

(4) None of these

Answer: 1


14. The equipment that is used to make clear magnified sketches of objects under a microscope is called?

(1) Compound microscope

(2) Light microscope

(3) Camera Lucida

(4) Camera attached stereomicroscope

Answer: 3


15. Which of the following is used in an electron microscope?

(1) electron beams

(2) magnetic fields

(3) light waves

(4) electron beams and magnetic fields

Answer: 4


16. Differentiation capacity of a compound microscope is________

(1) 27.5 μm

(2) 2.75 μm

(3) 0.275 μm

(4) None

Answer: 3


17. Resolving power of light microscope is________

(1) 2 mm

(2) 0.2 mm

(3) 0.1 mm

(4) 1 mm

Answer: 2


18. Which part of the compound microscope helps in gathering and focusing light rays on the specimen to be viewed?

(1) Eyepiece lens

(2) Objective lens

(3) Condenser lens

(4) Magnifying lens

Answer: 3


19. Which of the following is true about dark field microscopy?

(1) Adding a disc called ‘stop’ to the condenser will make a bright field to dark filed

(2) The stop disc prevents the entry of light from the central field and the object is illuminated with a beam of light

(3) The light gets reflected from the sides of the specimen and appears bright in the dark background

(4) All of these

Answer: 4


20. The magnification power of a compound microscope does not depend upon_______

(1) The focal length and aperture of the eye lens

(2)'The apertures of the objective lens

(3) Tube length of the microscope

(4) None of the above

Answer: 2


21. Resolving power of a microscope depends upon________

(1) The focal length and aperture of the eye lens

(2) The focal length and objective of the eye lens

(3) The apertures of the objective and the eye lens

(4) The wavelength of light illuminating the object

Answer: 4


22. Resolving power of a microscope is a function of ____________

(1) Wavelength of light used

(2) Numerical aperture of the lens system

(3) Refractive index

(4) Wavelength of light used and numerical aperture of the lens system

Answer: 4


23. In fluorescence microscopy, which of the following performs the function of removing all light except the blue light?

(1) Exciter filter

(2) Barrier filter

(3) Dichroic mirror

(4) Mercury arc lamp

Answer: 1


24. Which of the following is true for electron microscopy?

(1) specimen should be thin and dry

(2) image is obtained on a phosphorescent screen

(3) electron beam must pass through the evacuated chamber

(4) specimen should be thin and dry, image is obtained on a phosphorescent screen and electron beam must pass through evacuated chamber

Answer: 4 

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